Waters Alliance HPLC  

Waters Alliance HPLC THAT WORKS AS HARD AS YOU DO 
Confidence in your results comes with high performance, day-to-day reliability, and overall system robustness. The Alliance® HPLC System was designed to meet demanding method criteria for repeatability, linearity, and accuracy, resulting in data that you can depend on with eacy analysis. Achieving low carryover with a programmable wash routine, delivering reproducible gradients with automatic solvent compressibility and Auto•Blend™  Technology, and streamlining daily start-up with SystemPREP are just some of the ways that the Alliance HPLC System delivers the performance your methods require, making your laboratory more efficient.
Waters Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance H

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Waters Breeze 2 HPLC  

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs.
From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization seeking a quality HPLC platform with limited budget and chromatography experience including university laboratories, government laboratories, or start-up companies.
The Waters® Breeze 2 HPLC System delivers routine analyses and robust performance day after day, providing the  onfidence you need to get the job done. Used in laboratories worldwide, chromatograp

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Waters ACQUITY UPLC H-Class System  

With dispersion of less than 10 μL, the ACQUITY UPLC H-Class System delivers the highest resolution of any quaternary LC system in the market. Achieving true UPLC performance requires that system dispersion (or extra-column band spread) be matched to the peak widths associated with narrowbore columns packed with sub-2-μm particles.  While other systems might be designed for high pressure, it doesn’t mean they're designed to deliver the sharp peaks associated with UPLC performance and the ACQUITY UPLC H-Class System.
Highest resolution of any quaternary LC system

Improve your laboratory’s efficiency by scaling your methods to UPLC Technology
Use UPLC’s unmatched resolution to get better characterization of your complex samples
Take advantage of tools that simplify and str

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Waters ACQUITY Arc HPLC and UHPLC System  

HPLC and UHPLC method compatibility at the flip of a switch

The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support validated assays.

True plug-and-play method compatibility and the ability to switch between HPLC and UHPLC separations at the flip of a switch with Arc Multi-flow path Technology
Get mass detection, without changing the way you work, with the ACQUITY QDa Detector
Deploy the benefits of 2.x μm particle columns to boost the chromatographic performance of your methods
On-demand mobile phase creation with Auto●Blend Plus Technology, eliminating the cumbersome and error-prone appr

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principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

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Troubleshooting HPLC- Tailing Peaks  

As in the previous posts for this series, these suggestions assume that you are working with an established method and had successful results in the past. If a method is rugged, it is developed to minimize tailing by using a stationary column phase that is base-deactivated as well as appropriate modifiers and pH adjustment in the mobile phase as needed.  Keep in mind that if the method is not as rugged as it should be, change(s) in the method might be advised if tailing continues to be an ongoing problem.

With troubleshooting, as always, it is critical to note when the change occurred and how it might correlate to changes in the system.  Equally important is to change one thing at a time to identify the source(s) of difficulty. The following are examples of things that could contribute

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Features Of Agilent 1290 Infinity II LC System  

Maximize Analytical Efficiency
More chromatographic resolution – specially designed components in the sample flow path achieve lowest system dispersion.
Higher peak capacity for challenging separations ­– switch easily between single dimension UHPLC and the ultimate chromatographic power of 2D-LC.
Lower carryover for uncompromised data quality – multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine.
Unique detection capabilities - combine lowest detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR- DAD or 1290 Infinity II ELSD.
Maximize Instrument Efficiency
Faster injection cycles with dual-needle injection and smart overlapped injections off

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Agilent 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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Hplc Agilent Technologies 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
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Waters Alliance HPLC THAT WORKS AS HARD AS YOU

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